Estimation of Atenolol by Reverse Phase High Performance Liquid Chromatography
نویسندگان
چکیده
منابع مشابه
Reverse-phase chromatography of polar biological substances: separation of catechol compounds by high-performance liquid chromatography.
Catecholamines and their metabolites have been separated isocratically by reverse-phase chromatography with aqueous (no organic solvent admixed) eluents. Unlike ion-exchange or ion-pair chromatography, mixtures of both acidic and basic substances can be separated in a single chromatographic run, because the retention is governed by hydrophobic interactions between the nonpolar moiety of the sol...
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A rapid, simple and reproducible high performance liquid chromatographic method was developed and validated for determination of fluconazole in human plasma. The separation was performed on MZ C8 column (125 x 4 mm, 5 µm) using acetonitrile - potassium dihydrogen phosphate buffer (15 : 85, v/v), pH 3.0, as the mobile phase at a flow rate of 1.5 mL/min. The wavelength was set at 261 nm. The assa...
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In studies on endogenous plant gibberellins (GAs), reverse phase (Bondapak C(18)) high performance liquid chromatography (HPLC) has proved to be a useful method for the fractionation of plant extracts. The behavior of 18 authentic GAs in such a chromatographic system is described. The main factors determining chromatographic behavior are the degree and the position of hydroxylation of the GA. G...
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Original results and conclusions on Reverse Phase High Performance Liquid Chromatography (RPLC) mechanism are here presented. So far, none of the theoretical approaches applied to the RPLC mechanism can explain the retention and elution mechanisms of most of the analytes by RPLC, especially neutral organic compounds. Our experiences allowed us to state that RPLC retention mechanism most likely ...
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ژورنال
عنوان ژورنال: E-Journal of Chemistry
سال: 2010
ISSN: 0973-4945,2090-9810
DOI: 10.1155/2010/601650